Twitter Updates: December4-5, 2011
Kristy’s Posts: I love waking up to mornings like this!
Just watched Water for Elephants. What a beautiful movie I loved it. A hard journey that led to a happy ending. The passion is inspiring!
Sleep would be so good!
Liam’s Chimerism Results
Posted on December 6, 2011 by Nana
Liams graft results aren’t what we wanted:( CD3 Tcell 76% donor CD33 10% donor CD56 NK function 40% donor CD19 8% nothing seems 2 make sense
I have so much anger right now. I’m paralyzed with fear as the reality of this horrible disease really sinks in. U try 2 become emotionless
DAY+ 200 – 11/29/11 DNA Chimerism Analysis – Blood
(CD3+) T Cell 76% donor origin (24% host)
(CD33+) Myeloid Cells 10% donor origin (90% host)
(CD56+) NK Cells 40% donor origin (60% host)
(CD19+) B Cells 8% donor origin (92% host)
DAY+ 138 – 09/27/11 DNA Chimerism Analysis – Blood
(CD3+) T Cell 62% donor origin (38% host)
(CD33+) Myeloid Cells 23% donor origin (77% host)
(CD56+) NK Cells 71% donor origin (29% host)
(CD19+) B Cells 22% donor origin (78% host)
DAY +28 – 06/14/11 DNA Chimerism Analysis
(CD3+) T Cell 59% donor origin (41% host)
(CD33+) Myeloid Cells 100% donor origin (0% host)
(CD56+) NK Cells 100% donor origin (0% host)
(CD19+) B Cells 100% donor origin (0% host)
Chimerism analysis is important for monitoring post-transplant outcome and for determining appropriate therapy after transplantation. Since it is the basis for providing early treatment decisions, chimerism analysis requires sensitive and reliable techniques to detect the presence of donor leukocytes in the hosts’ peripheral blood or bone marrow. As lymphoid and myeloid engraftment is asynchronous, lineage-specific chimerism analysis is necessary. This requires the specific enrichment of myeloid and lymphoid cells prior to performing chimerism analysis (e.g. by PCR-based detection of donor- or recipient-specific mini-satellite DNA sequences).http://www.stemcell.com/en/Products/Area-of-Interest/Chimerism.aspx
Chimerism in nonmalignant diseases
http://www.nature.com/bmt/journal/v35/n2/full/1704715a.html
Allogeneic stem cell transplantation is the only curative treatment option for many patients with inherited or acquired nonmalignant diseases as thalassemia, sickle cell disease, immunodeficiency diseases, osteopetrosis, storage diseases, severe aplastic anemia, bone marrow failure syndromes and others (for a review, see Section IV in Thomas et al34). The aim of the procedure in these diseases is to achieve sustained engraftment to (i) improve the hematopoietic function, to (ii) correct the immune competence and/or to (iii) increase or normalize the respective enzyme shortage. Therefore a priori, it is not necessary to replace the recipient hematopoietic system completely. The implementation of a state of mixed chimerism is mostly sufficient to substantially improve the patient’s well being. Thus, to reduce toxic side effects, most conditioning regimens are less myeloablative and thereby mixed chimerism is more likely.60,61,62,63 As a consequence, graft rejection or nonengraftment remained the major causes of treatment failures in this diseases. Sensitization to minor histocompatibility antigens by prior blood product transfusion might increase this danger. The rapid development of complete chimerism in NK and T cells seems to play an important role in achieving sustained engraftment especially in patients who were treated with a dose reduced conditioning regimen.64,65,66,67
http://www.nature.com/bmt/journal/v35/n2/fig_tab/1704715t1.html#figure-title
Pediatric Transplants
Increasing mixed chimerism and the risk of graft loss in children undergoing allogeneic hematopoietic stem cell transplantation for non-malignant disorders
http://www.nature.com/bmt/journal/v42/n2/full/bmt200889a.html
http://www.nature.com/bmt/journal/v42/n2/pdf/bmt200889a.pdf
Please say a prayer for Little Ryan’s Family
Terri Smith posted in Liam’s Journey – HLH Warrior.
10:27am Dec 6
Our hearts are broken over the loss of Ryan and our hearts and prayers go out to his mommy and daddy , Holly and Dusty-xoxo He had just turned one, on the 9th and was 127+ post BMT, very unexpected, he had surgery to replace his hickman line yesterday due to infection. Not sure exactly what happened but know prayers are much needed. His family is from Oregon and need our prayers. You can write a note to his family on his caringbridge site.
http://www.caringbridge.org/visit/ryandavis
CaringBridge / Ryan Davis / Welcome
♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ ♥ Our hearts are broken, I’m in such shock. Holly Davis we are sending our love and prayers. ♥ ♥ RIP Ryan
Ryan had HLH, and had his BMT on July 29th, 2011
http://www.facebook.com/pages/
DAY +209
Kristy’s Twitter Update: I spoke with Liam’s dr in Seattle & he said not be worried that his t-cell has went up & that is most important. They will check again n Jan 
